Please login first
The antitumoral potential of Pllans-II, an acidic PLA2 from Porthidium lansbergii lansbergii snake venom on human cervical carcinoma cells.
* 1 , 1 , 2 , 3 , * 1
1  Grupo de Nutrición, Facultad de Salud, Universidad del Valle, Cali, Colombia
2  Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali, Colombia
3  Laboratory of Human Molecular Genetics, Department of Biology, Universidad del Valle, Cali, Colombia

Published: 14 January 2021 by MDPI in 1st International Electronic Conference on Toxins session Poster

Pllans-II, an Asp49-type acidic phospholipase A2 from Porthidium lansbergii lansbergii snake venom, displayed for the first time antitumoral potential against the squamous epithelial cell line of cervical cancer Ca Ski. Pllans–II presented a dose-dependent cytotoxic effect on cancer cells and an insignificant effect on healthy endothelial cells HUVEC. Pllans–II also inhibited the adhesion and migration ability of cancer cells and induced cell cycle arrest in the G2/M phase and apoptosis on Ca Ski cells. Transcriptomic analysis revealed that cell death was related to endoplasmic reticulum stress by interfering with α5 and β1–containing integrins. These results demonstrate that Pllans–II has an antitumor potential on cervical cancer and represents a possible biotechnological tool for designing anticancer prototypes.

Keywords: Cervical cancer; Cytotoxicity; Apoptosis; Endoplasmic reticulum stress; Phospholipase A2.