Pllans-II, an Asp49-type acidic phospholipase A₂ from Porthidium lansbergii lansbergii snake venom, displayed for the first time antitumoral potential against the squamous epithelial cell line of cervical cancer Ca Ski. Pllans–II presented a dose-dependent cytotoxic effect on cancer cells and an insignificant effect on healthy endothelial cells HUVEC. Pllans–II also inhibited the adhesion and migration ability of cancer cells and induced cell cycle arrest in the G2/M phase and apoptosis on Ca Ski cells. Transcriptomic analysis revealed that cell death was related to endoplasmic reticulum stress by interfering with α5 and β1–containing integrins. These results demonstrate that Pllans–II has an antitumor potential on cervical cancer and represents a possible biotechnological tool for designing anticancer prototypes.