Isolation, Characterization, and Application of Bacteriophages against Salmonella enterica.

¹ Laboratory of Microbial Biotechnology, Enzymatics and Biomolecules (LBMEB), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour km 6, PO Box 1177 Sfax 3018, Tunisia.
² Laboratory of Microbial Biotechnology, Enzymatics and Biomolecules (LBMEB), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour km 6, PO Box 1177 Sfax 3018, Tunisia. Astrum Biotech, Business incubator, Center of Biotechnology
³ Laboratory of Microbial Biotechnology, Enzymatics and Biomolecules (LBMEB), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour km 6, PO Box 1177 Sfax 3018, Tunisia Astrum Biotech, Business incubator, Center of Biotechnology o

Published: 30 January 2021 by MDPI in MOL2NET'21, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 7th ed. congress CHEMBIO.ORG-07: Org. Chem., Med. Chem., Mol. Biol., & Pharm. Industry Congress, Paris, France-Galveston, USA, 2021.

https://doi.org/10.3390/mol2net-07-09216

Abstract:

Salmonella infection is an important foodborne consumer health concern that can be mitigated during food processing. Bacteriophage therapy imparts many advantages over conventional chemical preservatives including pathogen specificity, natural derivation, potency, and providing a high degree of safety. The objective of this study aimed to isolate and characterize phages that effectively control Salmonella food contamination. A total of 36 bacteriophages infecting Salmonella enterica were isolated, tested at different pH ranging from 3 to 11, and at high temperatures from 37 ° C to 70 ° C, then tested against 11 strains in order to define their host range. The kinetics of phages have been studied in order to understand their lysis process. The genomic restriction profile of the isolated phages was interpreted following the action of 5 restriction enzymes (BamHI, EcoRI, HindIII, and EcoRV, and NdeI). Tests of the application of these phages were carried out on a food matrix in order to evaluate their ability to fight against Salmonella enterica. The results obtained are very encouraging, showing the possibility of using the bacteriophages isolated against Salmonella enterica, which can have a significant socio-economic impact.

Keywords: Bacteriophages, Salmonella, Food industry, Biological control.

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Humbert G. Díaz

18 January 2022

REVIEWWWERS-07 workshop question. Dear authors, thank your kind support to our conference. Now publication stage is closed and we opened our online post-publication review/discussion workshop. In case, you answer questions/make questions to other papers you are entitled to become a MOL2NET conference certified reviewer. In this context, we have some questions for you.

Have you published these results in some indexed journal?

Do you considered to develop an Artificial Intelligence / Machine Learning (AI/ML) model able to predict Bacteriophage-Bacteria Interactions (BBIs) and apply them to your data; i.e. predict interaction of your phages to other bacteria, or the BBIs or mutated phage strains re-designed by synthetic Biology?

Please, don't hesitate to contact us at mol2net.chair@gmail.com if you are open to collaborate in this sense.

Please, remember ti post also your comments/questions to other papers of this or other congresses/sessions inside our conference in order to promote discussion, instructions here: https://mol2net-07.sciforum.net/#reviewwwers