This work is a retrospective study of uterine lavage of mares with a history of sterility, sent by clinical veterinarians to the Infectious Pathology Department of the Clinical Veterinary Hospital of Cáceres during 2019.
The purpose of this study is to evaluate the prevalence of the main microorganisms isolated from uterine lavage of mares with reproductive problems, as well as the antimicrobial resistance of the different pathogens found.
A total of 66 samples were grown on selective and non-selective culture media and under different atmospheric conditions. The final identification was carried out by a commercial biochemical test (API; Biomerieux). Susceptibilities to different antibiotics were determined by Kirby-Bauer disk diffusion methods according to the Clinical Laboratory Standards Institute. The antimicrobials used were as follows: Ampicillin, Penicillin, Cefazolin, Ceftiofur, Cefquinome, Gentamicin, Amikacin, Enrofloxacin, Ciprofloxacin, Doxycycline and Trimethoprim/Sulfamethoxazole. All of them are widely used in equine clinics.
The results indicated a bacterial growth in 63.6% of the samples. Mixed growth (24.2%) was more frequent than pure growth. The most commonly isolated bacterial species was Escherichia coli (28.6%), followed by Streptococcus sp. (23.8%), Klebsiella sp. (15.9%) and Pseudomonas sp. (12.7%). On the other hand, fungi were isolated in 4.6% of them.
The main resistances observed in E. coli were to Doxycycline (44.4%), Ampicillin and Cefquinoma (38.9%), whereas the antibiotic to which they were most sensitive was Enrofloxacin (83.3%). In the case of Streptococcus sp., the most of these were resistant to Amikacin (100%) and Enrofloxacin (66.7%) and sensitive to Ceftiofur (88.7%). Klebsiella sp. was resistant to Ampicillin in all strains (100%) and Cefquinome (60%), and sensitive to Enrofloxacin (80%). Finally, Pseudomonas sp. showed high resistance to Ampicillin (75%) and Doxycycline and Trimethoprim / Sulfamethoxazole (62.5%) and showed a great sensitivity to Enrofloxacin (100%).
