The research Effect of Rhizobium inoculation on tomato (Solanum lycopersicum L.) yield in protected crops, was developed in a soft sialitic brown soil without carbonates, in a protected cultivation house "Campo Antena", belonging to the Integral Agropecuaria Company Santiago Cuba, from November 2018 to April 2019. The objective of this work was to evaluate the effect of different strains of Rhizobium on the yield of the Agean hybrid tomato variety. Direct inoculation of microorganisms was used at the time of sowing and transplantation, in a proportion of 10% with respect to the volume of the root ball. The experimental design was in randomized blocks, with four treatments and each one with four replications, an uninoculated control and three levels of the inoculation factor with the strains of Rhizobium, Rhizobium etli CE 3, Rhizobium leguminosarum SCR; Rhizobium leguminosarum Semia- 4088. The sampling was carried out in a zig zag throughout the field and the following variables were evaluated: dry mass by plant organs, foliar NPK, growth indicators, height and thickness of the plants, productive indicators, height, thickness and weight of the fruits, crop yield and economic valuation. The best results were obtained with the strain Rhizobium etli CE 3 (T2). The data were processed in the ANOVA statistical package, through a simple analysis of variance, applying Duncan's multiple comparison test of means for p≤5. The results obtained showed a positive effect regarding the variables evaluated in the plants inoculated with the Rhizobium strains with respect to the control without inoculation.