The present study was conducted to test for in vivo crown gall tumor disc bioassay using potato discs of the aqueous and ethanolic extracts of Annona reticulata with Allium sativum, Allium fistolisum Brassica oleraceae and correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of Crown Gall Tumor Disc Bioassay. The use of highly specific, quantitative bioassays which require only a short period of time to obtain results are available for studying crown gall tumor formation. Results showed that the extracts of Annona reticulata with Allium sativum, Allium fistolisum and Brassica oleraceae were potent against the Crown Gall Tumor Disc Bioassay when compared alone with combined extracts. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.