The Sponsors, IJMS, Cancers and Non-Coding RNA, offers one award to our participants at the conference:
1. Best Poster Award (1): 1 waived publication in one of our journals. The nominations have been assessed by the Organizing Committee.
Winner: Sara Terreri, Institute of Genetics and Biophysics (IGB-ABT), National Research Council (CNR), Naples, Italy
Sara Terreri the Winner of the 'Best Poster Award' together with George Calin, Chair ISFMS2017 and Franck Vazquez, CEO MDPI AG
Expression and Functional Characterization of Ultraconserved Non-Coding Regions 8+ in Bladder Cancer
Sara Terreri1, Sara Mancinelli1, Dimitrios Papaioannou2, Giovanna L. Liguori1, Ramiro Garzon2, Amelia Cimmino1
1 Institute of Genetics and Biophysics (IGB-ABT), National Research Council (CNR), Naples, Italy.
2 Division of Hematology, the Ohio State University, Comprehensive Cancer Center, Columbus, Ohio,
Transcribed ultraconserved regions (T-UCRs) represent a group of highly conserved sequences among orthologous regions of human, rat and mouse genomes. They represent a new class of long non-coding RNAs (lncRNAs) whose function is still unknown. While microRNAs and other types of lncRNAs have been shown to contribute to the biological function of bladder cancer (BlCa) and are increasingly being used to improve the clinical care of patients, this is not yet the case for T-UCRs. By using genome-wide profiling, we identified 293 T-UCRs de-regulated in BlCa patients as compared to normal tissues. T-UCR 8+ is the most up-regulated and inversely related to grade, paving the way for clinical applications. We demonstrated that T-UCR 8+ is localized in the cytoplasm of BlCa cells, suggesting that active molecular exportation could take place and be involved in cancer formation and/or progression. To better clarify the cytoplasmic function of T-UCR 8+ during tumorigenesis and understand its role, we dissect T-UCR 8+ protein network interaction using the RAP–MS method. As preliminary data, we purified T-UCR 8+ sequence and we are now performing the mass spectroscopy to verify which proteins bind to this lncRNA. To confirm its localization, we also performed ISH (In situ Hybridization) experiments in BlCa tissues and different embryo stages.