It is one of the great challenges to improve the sensitivity for early detection of biomarker in Alzheimer's disease (AD). In this work, an electrochemical aptasensor was prepared based on thionine (Th) - functionalized three - dimensional carbon nanomaterials (reduced graphene oxide (rGO) and multi-walled carbon nanotubes (MWCNTs) immobilized aptamer for the analysis of Aβ oligomers (AβO), one of the AD biomarkers. Th, the positively charged planar aromatic molecule, could form a large number of π - conjugated structures with rGO and MWCNTs, thereby bringing extra stability to the conjugated macromolecules and improve the structural stability and capacitive properties of Th-rGO-MWCNTs nanocomposites. Under the optimal conditions, DPV response decreased with the increase of AβO concentration. The aptasensor has a good linear range of 0.0443 pM to 443.00 pM with a LOD of 0.01 pM. It also has excellent stability and selectivity for AβO detection. This aptasensor thus has a great potential for early diagnosis of AD.