The Lunar Mission has involved with the different conditions from earth such as gravity, air pressure, temperature, lunar regolith and others. As a part of nuclear fusion project known in mini sun on earth, the Helium-3 is a fuel source from the moon. The clean energy will plan to use for extracting water, construction materials, fertilization, lunar agriculture and sustainable living in finally. The ore processing and extraction are needed to study more. The samples of lunar regolith have investigated from Apollo project until the created-prepared lunar regolith simulant from earth resource in various countries such as USA Europe India Russia and Thailand. The Thailand Lunar Simulant(TLS-01) in this research were reported the four parameters as physical properties, chemical composition, mineralogical characteristic, particle size distribution and the progress of mineral extraction by biomining method. The results found that the basalt rock from Trad province can use for the simulant material. The biomining can extract the necessary elements from the lunar regolith simulant which are Sodium Magnesium Iron Aluminum Titanium and Silica.