FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for murine liver tissues analysis

Martina Moggio; Sonia Errico; Nadia Diano; Maria Lepore

Abstract:

Over the past several decades, growing research on lipids and lipidomic technologies have shown how the perception of lipids has changed. Lipids are functionally versatile molecules in plants, animals and humans. They are certainly key components of the cell membranes and a source of energy, but they also play an essential role in physiology and pathophysiology, in signal transduction between cells and body metabolism and act as diagnostic and/or prognostic biomarkers of different diseases [1]. Many studies have shown the relationship between altered lipid metabolism and type 2 diabetes mellitus (T2DM) or metabolic disease as Nonalcoholic fatty liver disease (NAFLD) or neurodegenerative disease as Parkinson’s Disease or Atherosclerosis (a risk factor for ischemic stroke) [2-4]. A powerful technique used for lipids detection and characterization in biological tissues is Fourier Transform Infrared (FTIR) spectroscopy [5]. The main goal of the present work is to exploit FTIR spectroscopy as a tool for monitoring lipid extraction efficiency by evaluating three different lipid extraction methods [6]. FTIR spectroscopy is used to monitor the extraction efficiency of the Folch [7], Bume [8] and modified Bume [9] methods in murine liver tissues. In particular, infrared spectra will be obtained in the 4000-600 cm^-1 wavenumber region and the contributions of different functional groups will be evidenced. The ratio values estimated using the absorbance of selected bands related to different liver constituents will be used for a quantitative comparison of the efficiency of the different extraction methods.

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