The purpose of this investigation was to assess the influence of effective microorganisms on the growth and nutrient content of tomato transplants. There were two treatments: 1. with effective microorganism’s (EM) treatment; 2. without effective microorganism’s treatment – control. The height of tomato transplants was higher in control treatment compared to EM treatment. The stem diameter of tomato transplants was larger in EM treatment compared to control variant. The nutrient content of tomato leaves was very good. The contents of nitrates, N, P, K, Ca and Mg were higher in EM treatment compared to control treatment.