In order to study the effect of combined fertilizer management of bio-fertilizer and chemical fertilizers on grain yield of two dill (Anethum graveolens L.) ecotypes, a field experiment was arranged in a factorial layout based on randomized complete block design with three replications in 2019 in Agricultural Research Farm of the University of Tabriz. The first factor includes two ecotype (C₁: Native of Tabriz and C₂: Varamin); while the second factor was composed of five fertilizers levels, namely control (N₀), chemical fertilizers (N₁), Enterobactercloacae S16-3 bacteria + half a chemical fertilizer (N₂), Piriformospora indica Fungi + half a chemical fertilizer (N₃) and combination of bacteria + fungi + chemical fertilizers (N₄). Although the highest yield and yield components were obtained for chemical fertilizers treatment (N₁), but there was no statistically significant difference with the combined treatment (N₄). The results also showed that the highest grain yield were obtained for N₄.The combined application of biofertilizers (growth-promoting bacteria and fungi) in addition to reducing chemical fertilizers (50%), led to high grain yield. Accordingly, the application of combined treatment (N₄) can be a suitable treatment for the cultivation of medicinal plants, including dill.