The present study aims towards the Eco friendly (Green) Synthesis of gold nano particle (AuNPs) and study their Pharmacological activity. Gold has good ductlity, moleculer recognition properties and good biocompatibility. At present gold is being used in many fields. GNP physical and chemincal properties vary with their size in nanometers. The surface area of a nanosized gold surface has a special effect. Therefore gold nanoparticle can directly and indirectly, give rise to different biological activities. The sulfided Gold substances have a strong Chemical reactivity and are easy to combine with Sulfhydryl groups, hence nanogold is often used in biomedical testing, disease, diagnosis and gene detection. Nanogold binds to proteins as antibodies, enzymes or cytokines. In fact, Scientist use nonogold to bind Special antibodies, as a tool for targeting Cancer cells. Gold name particles are also directly cytotoxic to cancer cells. For diseases caused by inflammation and oxidative damage, AuNPs also have antioxidant and anti-inflamatory effects. Based on these unique properties, AuNPs have become the most widely studied metal nanomaterials. Many recent Studies have further demonstrated that AuNPS are beneficial for humans, due to their functional Pharmacological properties in variety of diseases. In this study, we report a novel method of gold nonoparticle (AuNPs) synthesis using aqueous extract of leaves of Aloe barbadensis miller (Aloe vera). The phytochemicals present in the plant extract (PE) act as an effective reducing and Capping agent to synthesize AuNPs. The use of PE for green sythesis are cost effective and eco-friendly. The synthetized AuNPs were Characterised by Specto photometry, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy.The XRD patterns showed peaks at (111), (200), (220) which exhibited preferential orientation of the AuNPs as face Centred cubic crystal. FTIR measurements Confirmed the Coating of phonilic compounds on the AuNPs indicating a possible role of biomolecules for the Capping and efficient Stabilization of the AuNPs. UV- visible Spectra also showed peak at 540 nm & characteristics of AuNPS.