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Thymol elicitation during in vitro regeneration of axillary bud explants from a Thymus piperella L. commercial hybrid
Roberto de Medinacelli Juan-Méndez
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Academic Editor: Francisco Torrens
Published:
15 June 2022
by MDPI
in MOL2NET'22, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 8th ed.
congress CHEMBIO.ORG-08: Org. Chem., Med. Chem., Mol. Biol., & Pharm. Industry Congress, Paris, France-Galveston, USA, 2022.
Abstract:
Species of the genus Thymus L. (Lamiaceae) are of great interest as medicinal plants, as well as in the extracts and food technology industries due to the richness in bioactive specialized metabolites. Specifically, Iberian endemic species such as Th. piperella L., have a wide range of traditional uses in this sense. However, the micropropagation and biosynthesis of high-added value compounds under in vitro culture conditions has not been studied for this species and its commercial hybrids yet. Therefore, the main objective of this work was to develop a micropropagation protocol of a popularly used commercial hybrid of Thymus. The results obtained showed a good capacity of adaptation and initiation of in vitro culture after the sterilization treatment applied. In addition, the different plant development parameters measured in the multiplication phase showed significantly higher values in Murashige and Skoog (MS) medium supplemented with 0.013 mg/L 2-isopentenyladenine (2iP), or without plant growth regulators, plus 30 g/L sucrose and solidified with 5.5 g/L Plant Agar when compared to the other treatments applied. Finally, the test performed on the content of volatile compounds by HS-SPME-GC/MS revealed that there was a remarkable increase of thymol in the in vitro cultivated plants when compared to the samples from the wild-type plants. Therefore, the results obtained in the present work could be the basis for the development of future studies on the elicitation of this compound by in vitro culture systems with this hybrid, or other plant materials from Thymus piperella chemotypes
Keywords: in vitro culture; Thymus; terpene; thymol, plant growth regulator; microrpopagation
Comments on this paper
estefania Ascencio
4 January 2023
Dear authors thank you for your support to the conference.
Now we closed the publication phase and launched the post-publication phase of the conference. REVIEWWWERS'08 Brainstorming Workshop is Now Open from 2023-Jan-01 to 2023-Jan-31. MOL2NET Committee, Authors, and Validated Social Media Followers Worldwide are ... Invited to Post Moderated Questions/Answers, Comments, about papers. Please kindly post your public Answers (A) to the following questions in order to promote interchange of scientific ideas. These are my Questions (Q) to you:
Q1. What could be some of the limitations that were found in this study regarding the techniques used, information, etc.
Q2. Considering that the species of the genus Thymus L. (Lamiaceae), has a great interest at industrial level. Have you considered and/or are you in process of creating a similar startup company with students of your college?
Now the conference publication phase is close but you can answer here directly as a post-publication comment. However, in case you want to elaborate your answers in a more structured way in the form of a short communication we can upload it free-of-cost on your behalf to the CATCHTOHIT-03: Congress on Acad. Transl. Res. for Companies Helping to Height. Innov. & Tech., Bilbao-Cambridge, MA, USA, 2022. https://mol2net-08.sciforum.net/catchtohit-03
Thank you for your kind support. Please make questions to other papers in different Mol2Net congresses
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Now we closed the publication phase and launched the post-publication phase of the conference. REVIEWWWERS'08 Brainstorming Workshop is Now Open from 2023-Jan-01 to 2023-Jan-31. MOL2NET Committee, Authors, and Validated Social Media Followers Worldwide are ... Invited to Post Moderated Questions/Answers, Comments, about papers. Please kindly post your public Answers (A) to the following questions in order to promote interchange of scientific ideas. These are my Questions (Q) to you:
Q1. What could be some of the limitations that were found in this study regarding the techniques used, information, etc.
Q2. Considering that the species of the genus Thymus L. (Lamiaceae), has a great interest at industrial level. Have you considered and/or are you in process of creating a similar startup company with students of your college?
Now the conference publication phase is close but you can answer here directly as a post-publication comment. However, in case you want to elaborate your answers in a more structured way in the form of a short communication we can upload it free-of-cost on your behalf to the CATCHTOHIT-03: Congress on Acad. Transl. Res. for Companies Helping to Height. Innov. & Tech., Bilbao-Cambridge, MA, USA, 2022. https://mol2net-08.sciforum.net/catchtohit-03
Thank you for your kind support. Please make questions to other papers in different Mol2Net congresses
Commenting Steps: Login, Go to Papers List, Select Paper, Write Comment, Click Post Comment
Papers list: https://mol2net-08.sciforum.net/presentations/view
Workshop link: https://mol2net-08.sciforum.net/#reviewwwers
Jorge Juan-Vicedo
5 January 2023
Dear Estefania,
Thank you very much for your questions. Please, find attached the answers beneath:
The study that we presented here is a preliminary experience on "in vitro behavior" of this hybrid that, moreover, had not been studied before. Therefore the main limitations are related to the current low amount of data available (including the humble data that we obtained in this preliminary work). For instance, more plant growth regulators should be studied (including elicitors such as MeJa), as well as a wider range of concentrations, and (ideally) different in vitro culture systems (bioreactors, liquid media, etc.) or morphogenic pathways (callus induction for cell suspension culture development) should be applied in order to optimize the micropropagation rate, and to obtain a more complete view on the in vitro biosynthesis (and elicitation) of these valuable compounds (mainly thymol). In this sense, complementary analytical techniques must be also used (we only used HS-SPME-GC/MS, but organic extracts and essential oils characterization by GC/MS are needed) in order to have a better perspective of the chemical profile of the plant. Some of these issues were further undertaken in our research group, and new interesting information was obtained (If you wish to know more, please, check the paper that we recently published on this -> https://doi.org/10.3390/horticulturae8121188). For instance, thymol was also quantified by GC/MS (from methanol extracts) and it was revealed as the major compund, in quite high concentrations, what supports what we estimated before by HS-SPME-GC/MS, and makes this plant quite promising for the exploitation of thymol. In contrast, variations on thymol biosynthesis under in vitro conditions were not statistically different when compared to the donnor plants (unlike other compounds) according to the new experiments made (that comprised new replicates, and complementary analytical techniques). However, biosynthesis of all analyzed compounds was restored after transference microplants to ex vitro conditions (this not always happens). This finding indicates that true-to-type plants can be obtained (and thymol can be obtained from micropropagated plants), but elicitation under in vitro conditions is not clear at all (at least, under the experimental conditions that we designed). For all these reasons, we will try in a near future other in vitro systems in order to check alternative ways of elicitation. All these data are necessary before thinking about setting up a start up or any bussiness with this plant, but we will be glad in doing it if we find plant materials that can be industrially exploited.
Thank you very much for your questions. Please, find attached the answers beneath:
The study that we presented here is a preliminary experience on "in vitro behavior" of this hybrid that, moreover, had not been studied before. Therefore the main limitations are related to the current low amount of data available (including the humble data that we obtained in this preliminary work). For instance, more plant growth regulators should be studied (including elicitors such as MeJa), as well as a wider range of concentrations, and (ideally) different in vitro culture systems (bioreactors, liquid media, etc.) or morphogenic pathways (callus induction for cell suspension culture development) should be applied in order to optimize the micropropagation rate, and to obtain a more complete view on the in vitro biosynthesis (and elicitation) of these valuable compounds (mainly thymol). In this sense, complementary analytical techniques must be also used (we only used HS-SPME-GC/MS, but organic extracts and essential oils characterization by GC/MS are needed) in order to have a better perspective of the chemical profile of the plant. Some of these issues were further undertaken in our research group, and new interesting information was obtained (If you wish to know more, please, check the paper that we recently published on this -> https://doi.org/10.3390/horticulturae8121188). For instance, thymol was also quantified by GC/MS (from methanol extracts) and it was revealed as the major compund, in quite high concentrations, what supports what we estimated before by HS-SPME-GC/MS, and makes this plant quite promising for the exploitation of thymol. In contrast, variations on thymol biosynthesis under in vitro conditions were not statistically different when compared to the donnor plants (unlike other compounds) according to the new experiments made (that comprised new replicates, and complementary analytical techniques). However, biosynthesis of all analyzed compounds was restored after transference microplants to ex vitro conditions (this not always happens). This finding indicates that true-to-type plants can be obtained (and thymol can be obtained from micropropagated plants), but elicitation under in vitro conditions is not clear at all (at least, under the experimental conditions that we designed). For all these reasons, we will try in a near future other in vitro systems in order to check alternative ways of elicitation. All these data are necessary before thinking about setting up a start up or any bussiness with this plant, but we will be glad in doing it if we find plant materials that can be industrially exploited.
Jorge Juan-Vicedo
5 January 2023
Dear Estefania,
Thank you very much for your questions. Please, find attached the answers beneath:
The study that we presented here is a preliminary experience on "in vitro behavior" of this hybrid that, moreover, had not been studied before. Therefore the main limitations are related to the current low amount of data available (including the humble data that we obtained in this preliminary work). For instance, more plant growth regulators should be studied (including elicitors such as MeJa), as well as a wider range of concentrations, and (ideally) different in vitro culture systems (bioreactors, liquid media, etc.) or morphogenic pathways (callus induction for cell suspension culture development) should be applied in order to optimize the micropropagation rate, and to obtain a more complete view on the in vitro biosynthesis (and elicitation) of these valuable compounds (mainly thymol). In this sense, complementary analytical techniques must be also used (we only used HS-SPME-GC/MS, but organic extracts and essential oils characterization by GC/MS are needed) in order to have a better perspective of the chemical profile of the plant. Some of these issues were further undertaken in our research group, and new interesting information was obtained (If you wish to know more, please, check the paper that we recently published on this -> https://doi.org/10.3390/horticulturae8121188). For instance, thymol was also quantified by GC/MS (from methanol extracts) and it was revealed as the major compund, in quite high concentrations, what supports what we estimated before by HS-SPME-GC/MS, and makes this plant quite promising for the exploitation of thymol. In contrast, variations on thymol biosynthesis under in vitro conditions were not statistically different when compared to the donnor plants (unlike other compounds) according to the new experiments made (that comprised new replicates, and complementary analytical techniques). However, biosynthesis of all analyzed compounds was restored after transference microplants to ex vitro conditions (this not always happens). This finding indicates that true-to-type plants can be obtained (and thymol can be obtained from micropropagated plants), but elicitation under in vitro conditions is not clear at all (at least, under the experimental conditions that we designed). For all these reasons, we will try in a near future other in vitro systems in order to check alternative ways of elicitation. All these data are necessary before thinking about setting up a start up or any bussiness with this plant, but we will be glad in doing it if we find plant materials, and procedures that can be industrially exploited.
Thank you very much for your questions. Please, find attached the answers beneath:
The study that we presented here is a preliminary experience on "in vitro behavior" of this hybrid that, moreover, had not been studied before. Therefore the main limitations are related to the current low amount of data available (including the humble data that we obtained in this preliminary work). For instance, more plant growth regulators should be studied (including elicitors such as MeJa), as well as a wider range of concentrations, and (ideally) different in vitro culture systems (bioreactors, liquid media, etc.) or morphogenic pathways (callus induction for cell suspension culture development) should be applied in order to optimize the micropropagation rate, and to obtain a more complete view on the in vitro biosynthesis (and elicitation) of these valuable compounds (mainly thymol). In this sense, complementary analytical techniques must be also used (we only used HS-SPME-GC/MS, but organic extracts and essential oils characterization by GC/MS are needed) in order to have a better perspective of the chemical profile of the plant. Some of these issues were further undertaken in our research group, and new interesting information was obtained (If you wish to know more, please, check the paper that we recently published on this -> https://doi.org/10.3390/horticulturae8121188). For instance, thymol was also quantified by GC/MS (from methanol extracts) and it was revealed as the major compund, in quite high concentrations, what supports what we estimated before by HS-SPME-GC/MS, and makes this plant quite promising for the exploitation of thymol. In contrast, variations on thymol biosynthesis under in vitro conditions were not statistically different when compared to the donnor plants (unlike other compounds) according to the new experiments made (that comprised new replicates, and complementary analytical techniques). However, biosynthesis of all analyzed compounds was restored after transference microplants to ex vitro conditions (this not always happens). This finding indicates that true-to-type plants can be obtained (and thymol can be obtained from micropropagated plants), but elicitation under in vitro conditions is not clear at all (at least, under the experimental conditions that we designed). For all these reasons, we will try in a near future other in vitro systems in order to check alternative ways of elicitation. All these data are necessary before thinking about setting up a start up or any bussiness with this plant, but we will be glad in doing it if we find plant materials, and procedures that can be industrially exploited.
Jorge Juan-Vicedo
5 January 2023
Dear Estefania,
Thank you very much for your questions. Please, find attached the answers beneath:
The study that we presented here is a preliminary experience on "in vitro behavior" of this hybrid that, moreover, had not been studied before. Therefore the main limitations are related to the current low amount of data available (including the humble data that we obtained in this preliminary work). For instance, more plant growth regulators should be studied (including elicitors such as MeJa), as well as a wider range of concentrations, and (ideally) different in vitro culture systems (bioreactors, liquid media, etc.) or morphogenic pathways (callus induction for cell suspension culture development) should be applied in order to optimize the micropropagation rate, and to obtain a more complete view on the in vitro biosynthesis (and elicitation) of these valuable compounds (mainly thymol). In this sense, complementary analytical techniques must be also used (we only used HS-SPME-GC/MS, but organic extracts and essential oils characterization by GC/MS are needed) in order to have a better perspective of the chemical profile of the plant. Some of these issues were further undertaken in our research group, and new interesting information was obtained (If you wish to know more, please, check the paper that we recently published on this -> https://doi.org/10.3390/horticulturae8121188). For instance, thymol was also quantified by GC/MS (from methanol extracts) and it was revealed as the major compund, in quite high concentrations, what supports what we estimated before by HS-SPME-GC/MS, and makes this plant quite promising for the exploitation of thymol. In contrast, variations on thymol biosynthesis under in vitro conditions were not statistically different when compared to the donnor plants (unlike other compounds) according to the new experiments made (that comprised new replicates, and complementary analytical techniques). However, biosynthesis of all analyzed compounds was restored after transference microplants to ex vitro conditions (this not always happens). This finding indicates that true-to-type plants can be obtained (and thymol can be obtained from micropropagated plants), but elicitation under in vitro conditions is not clear at all (at least, under the experimental conditions that we designed). For all these reasons, we will try in a near future other in vitro systems in order to check alternative ways of elicitation. All these data are necessary before thinking about setting up a start up or any bussiness with this plant, but we will be glad in doing it if we find plant materials, and procedures that can be industrially exploited.
Thank you very much for your questions. Please, find attached the answers beneath:
The study that we presented here is a preliminary experience on "in vitro behavior" of this hybrid that, moreover, had not been studied before. Therefore the main limitations are related to the current low amount of data available (including the humble data that we obtained in this preliminary work). For instance, more plant growth regulators should be studied (including elicitors such as MeJa), as well as a wider range of concentrations, and (ideally) different in vitro culture systems (bioreactors, liquid media, etc.) or morphogenic pathways (callus induction for cell suspension culture development) should be applied in order to optimize the micropropagation rate, and to obtain a more complete view on the in vitro biosynthesis (and elicitation) of these valuable compounds (mainly thymol). In this sense, complementary analytical techniques must be also used (we only used HS-SPME-GC/MS, but organic extracts and essential oils characterization by GC/MS are needed) in order to have a better perspective of the chemical profile of the plant. Some of these issues were further undertaken in our research group, and new interesting information was obtained (If you wish to know more, please, check the paper that we recently published on this -> https://doi.org/10.3390/horticulturae8121188). For instance, thymol was also quantified by GC/MS (from methanol extracts) and it was revealed as the major compund, in quite high concentrations, what supports what we estimated before by HS-SPME-GC/MS, and makes this plant quite promising for the exploitation of thymol. In contrast, variations on thymol biosynthesis under in vitro conditions were not statistically different when compared to the donnor plants (unlike other compounds) according to the new experiments made (that comprised new replicates, and complementary analytical techniques). However, biosynthesis of all analyzed compounds was restored after transference microplants to ex vitro conditions (this not always happens). This finding indicates that true-to-type plants can be obtained (and thymol can be obtained from micropropagated plants), but elicitation under in vitro conditions is not clear at all (at least, under the experimental conditions that we designed). For all these reasons, we will try in a near future other in vitro systems in order to check alternative ways of elicitation. All these data are necessary before thinking about setting up a start up or any bussiness with this plant, but we will be glad in doing it if we find plant materials, and procedures that can be industrially exploited.
