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BSA‐PEG hydrogel: A novel protein‐ligand binding 3D matrix
* 1 , 1 , 1, 2 , 3 , 1
1  Coimbra Chemistry Center, Chemistry Department, University of Coimbra, Coimbra, Portugal
2  Health Research Unit and School of Health Sciences, Polytechnic Institute of Leiria, Leiria, Portugal
3  School of Technology and Management, Polytechnic Institute of Leiria, Leiria, Portugal
Academic Editor: Mário Diniz


Hydrogel materials have good biomimetic properties and high potential for biomedical and bioanalytical applications. In this work, an hydrogel of serum albumin crosslinked with poly-(ethylene glycol) was prepared and characterized for its water content, protein structure and stability. The ability of the hydrogel to bind small molecule ligands with different hydrophobicity was evaluated using a homologous series of amphiphiles (NBD-Cn, n=4, 6 and 8) and the calculated binding affinities were similar to that of free protein in solution. Overall, the results indicate this type of hydrogel system as convenient tools for studying the binding of xenobiotics to tissue proteins.

Keywords: Hydrogel ; Binding Constant ; BSA ; NBDs ; Toxic Small Molecules