Many chemicals and methods have been extensively studied for the removal of copper, chromium, and arsenic from wood treated with chromate copper arsenate (CCA). However, in these studies, it is seen that deep eutectic solvents (DES) are not used in the removal of copper, chromium, and arsenic. In this study, the effect of DES on the removal of copper, chromium, and arsenic from CCA-impregnated wood and the changes caused by DES solutions in wood samples were investigated. The DES solutions were prepared by mixing glycerol (GL) as the hydrogen bond donor (HBD) and choline chloride as the hydrogen bond acceptor (HBA) in different molar ratios. According to the results obtained, it was observed that the LA solution prepared with GL: CL was removed 65.5% Cu, 40.21% Cr, and 15.20% As. It was obtained in FTIR analysis and lignin determination that the lignin ratio of the wood samples decreased after the remediation process. It seems that the crystallinity ratio decreased significantly. The physical and biological properties of the sheet were investigated by making a sheet from powder samples treated with DES solutions. According to the results obtained, while the brightness values of the samples did not change, the roughness values decreased. In addition, the water uptake values of the samples after 96 hours showed a decrease of approximately 40-50% compared to the control samples. After the decay test, the weight loss values decreased compared to the control samples.