In dairy sector, mastitis is one of the most important and costly disease because due to it the world’s economy faces about 35 billion dollar loss each year. Bacterial pathogens that cause mastitis are called mastitogens. Among these bacterial species Escherichia coli, Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactia and Streptococcus dysgalactia are the most important. Mastitis is treated with antibiotics however; due to their improper, excessive and irrational usage, these pathogens have become resistant to them. Drug residues in milk is also a factor resulting in multi-drug resistance (MDR) in mastitogens that cause treatment to become ineffective. In Pakistan, several ethnoveterinary plants such as Allium sativum (Garlic) and Chenopodium album (Goosefoot) are used for the treatment of mastitis in cattle and buffalo. For this purpose, we prepared aqueous and methanol extracts of A. sativum and C. album. In the agar well diffusion method, the aqueous A. sativum showed strong activity against Staphylococcus aureus i.e. 20mm. whereas, the methanolic extract of C. album gave 11mm zone of inhibition against Staphylococcus aureus. By combining the extracts of A. sativum and C. album then they give a synergistic effect especially against E.coli. Results showed that zone of inhibition against Staphylococcus aureus was 16mm, against E.coli 22mm and against Streptococcus uberis was 5mm. Our study is in agreement with the use of A. sativum and C. album in cases of mastitis and recommend their combined use for better results.
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