Adansonia digitata L. (Malvaceae, Baobab) is a medicinal tree with antimicrobial, antiviral, anti-inflammatory, and antioxidant properties. The leaves, fruit pulp, stem bark, and roots have been extensively studied. The aim of this study was to evaluate the glucose-lowering and in vitro antidiabetic potentials of the aqueous extract of A. digitata seed. The aqueous extract of A. digitata seed was prepared by dissolving 50 g of powder seed in 500 mL of distilled water for 24 hours, filtered using Whatman filter paper, and concentrated using a rotary evaporator at 40^oC. Following an oral administration of glucose (2 g/kg body weight), distilled water, metformin (14.2 mg/kg body weight), and A. digitata seed extracts at 500 and 1000 mg/kg body weight respectively. Results show that the untreated mice had an average of 11.09% increase in plasma glucose concentration, while metformin, aqueous seed extract of A. digitata had an average of 17.05%, 0.99%, and 19.21% decrease in plasma glucose concentration respectively. The aqueous seed extract of A. digitata inhibited α amylase in a concentration-dependent manner with an IC₅₀ of 24.27±2.14 mg/mL compared with acarbose with IC₅₀ of 22.61±1.05 mg/mL. However, the α-glucosidase inhibitory activities of the extract (IC₅₀ 34.37±1.67 mg/mL) were significantly lower compared to acarbose (IC₅₀ 53.46±2.06). The study concludes that aqueous seed extract of A. digitata possesses glucose-lowering properties, in vitro α-amylase and α-glucosidase inhibitory potentials.