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Stability and in vitro biosafety study of an emulsion of Calendula officinalis L. with potential application in treating skin burn wounds
* 1 , 1 , 1 , 1 , 2
1  Biomedical Engineering Department, Universidad de los Andes, Bogotá, Colombia
2  Grupo de Diseño de Productos y Procesos (GDPP) Departamento de Ingeniería Química y de Alimentos, Universidad de los Andes, Bogotá, Colombia
Academic Editor: Vladimir Uversky


Skin burns are injuries of different degrees of complexity (first to third) caused by physical and/or chemical trauma. There is a necessity to improve the rapid retrieval of superficial wounds (first grade) to enhance epithelization, avoiding dehydration, infections, and scar formation. This work proposes an oil in water (O/W) emulsion based on 1% of Calendula officinalis L. extract, due its recognized traditional uses as medicinal plant in wound care, with reported beneficial secondary metabolites such as Carotenoids, Terpenoids, Flavonoids, Coumarins and Quinones. The rheologi-cal characterization of the obtained emulsions indicated superior stability over time (2 months) and a pseudoplastic and semisolid fluid behavior. Furthermore, the emulsions were evaluated biologi-cally in terms of biosafety in vitro with promising results that showed a non-hemolytic behavior (below 14.53%) and a moderate platelet aggregation (33.66%) tendency, which is beneficial as it can contribute to enhancing the healing process. Additionally, the emulsions were characterized physi-cochemically by frequency curve, flow curves of shear stress, and viscosity. Currently, we are con-ducting a droplet size distribution assay, and conducting 2D wound healing assays in a scratch model over a monolayer of keratinocytes. Thus far, the results hold much promise and indicate that these emulsions can be potentially employed in the treatments of burn wounds.

Keywords: Calendula officinalis L.; burns; emulsion.