Since December 2019, the problem caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has grown to a global threat. The search for new treatment strategies is strongly associated with both fundamental research on mechanisms of virus life cycle and development of new screening platforms for anti-viral drug candidates. In this project we labelled SARS-CoV-2 membrane proteins M, E, S and studied their localization in mammalian cell compartments using fluorescent microscopy. We tested N- and C-oriented sensor designs and different fluorescent proteins. Also, we successfully visualized the early stages of M protein transport in real time. We found that M protein localizes in cell lysosomes, which supports the recent hypothesis that β-coronaviruses use lysosomal organelles for egress instead of traditional Golgi-mediated secretory pathway. Further we plan to study interactions between M, E and S using the FRET method. In addition, we developed several types of FRET-based and translocational sensors to track SARS-CoV-2 PLpro protease and measure its activity in living cells. Preliminary experiments showed the expected increase in donor fluorescence after proteolysis of PLpro site between the FRET-pair. Results of the current project will provide unique information on spatial-temporal dynamics and interaction between SARS-CoV-2 membrane proteins during the viral lifecycle. The developed system for real-time visualization of PLpro activity can potentially serve as a basis for safe cell antiviral drug screening platforms. The proposed strategy for studying viral proteins combines two important properties. Firstly, research is conducted on human living cells, which is closely approximated to native conditions in contrast to in vitro experiments. Secondly, the experimental system lacks interaction with a functional virus which makes it completely safe for the researcher.
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Genetically encoded fluorescent probes for imaging of intracellular localization and activity of SARS-CoV-2 proteins
Published: 01 November 2022 by MDPI in The 2nd International Electronic Conference on Biomolecules session Biomacromolecules: Proteins
Keywords: SARS-CoV-2; coronavirus; COVID-19; genetically encoded probes; live cell imaging; FRET; fluorescence microscopy