Enterococcus spp are microorganisms described in the literature as the main cause of endocarditis and bacteremia, which are both severe conditions that can end the life of the patient, has a morbidity between 5 and 12% of cases and a mortality rate 23-46%; Its rapid dissemination, both intrahospital and interhospital, with a clonal expansion of pathogenic and antimicrobial-resistant strains, makes this situation worrying in the province of Villa Clara, where it also represents a serious problem and the number of patients who have presented Enterococcus in blood cultures, as there is no research on this subject. Therefore, there is a knowledge gap in the matter and it is necessary to deepen the epidemiology and microbiological characteristics of the Enterococcus isolates circulating in the different hospitals of the province of Villa Clara. In this project we are going to focus / pursue the following objectives. Scientific Problem: Determining what microbiological characteristics will the Enterococcus isolated in blood cultures of different provincial hospitals of Villa Clara have. General Objective: Characterize, according to microbiological aspects, Enterococcus spp. Isolates in blood cultures of patients admitted to the different provincial hospitals of Villa Clara, in the period January - December 2022. Specific Objectives: Describe cultural characteristics of the Enterococcus isolates in the period of study. Describe microscopic characteristics of the colonies. Determine the species of Enterococcus to obtain according to hospitals and services studied. Identify susceptibility of Enterococcus against tested antimicrobials according to Clinical and Laboratory Standards Institute (CLSI).