Variances in the Expression of mRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer

¹ Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academy of Silesia, 41-800 Zabrze, Poland
² Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, Academy of Silesia, 40-055 Katowice, Poland
³ Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, 41-800 Zabrze, Poland
⁴ Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland
⁵ Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, Academy of Silesia, 40-555 Katowice, Poland
⁶ Department of Neurosurgery, 5th Military Clinical Hospital with the SP ZOZ Polyclinic in Krakow, 30-901 Krakow, Poland

Academic Editor: Humbert G. Díaz

Published: 03 April 2023 by MDPI in MOL2NET'23, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 9th ed. congress CHEMBIO.MOL-09: Org. Chem., Med. Chem., Mol. Biol., & Pharm. Industry Congress, Paris, France-Fargo, USA, 2023.

https://doi.org/10.3390/mol2net-09-14328 (registering DOI)

Abstract:

Research has indicated higher concentrations of histamine and polyamine in endometrioid tissue in comparison with healthy tissue. The aim of this study was to evaluate changes in the expression patterns of messenger RNA (mRNAs) and microRNA (miRNAs) related to the histaminergic system in endometrial samples and whole blood in women with endometrioid endometrial cancer. The study group consisted of 30 women with endometrioid endometrial cancer qualified for hysterectomy (G1 well-differentiated, 15 cases; G2 moderately differentiated, 8 cases; and G3 poorly differentiated, 7 cases). The control group included 30 women with no neoplastic changes during routine gynecological examinations. The molecular analysis consisted of the microarray analysis of mRNAs and miRNAs related to the histaminergic system, reverse-transcription quantitative polymerase chain reaction (RTqPCR), and enzyme-linked immunosorbent assay (ELISA). Out of 65 mRNAs connected with the histaminergic system, 10 differentiate the samples of tissue and blood obtained from patients with endometrioid endometrial cancer in comparison with the control group (p < 0.05). mRNA histamine receptor 1,3 (HRH1, HRH3), and solute carrier family 22 member 3 (SLC23A2) differentiating samples of endometrioid endometrial cancer independent of either G or control. The selected mRNA transcripts seem to be promising for molecularly targeted therapies in the context of endometrioid endometrial cancer.

Keywords: endometrioid endometrial cancer, grading, histaminergic system, molecular marker

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Maider Baltasar Marchueta

4 January 2024

Dear authors thank you for your support to the conference.

Now we closed the publication phase and launched the post-publication phase of the conference. REVIEWWWERS Brainstorming Workshop is now open until January 25th. MOL2NET committee, authors, and social media followers worldwide are invited to post questions/answers and comments about papers. Please kindly post your public Answers (A) to the following questions in order to promote interchange of scientific ideas.

My Questions (Q) to you:
Q1. In the context of the study, could you elaborate on the significance of evaluating both endometrial samples and whole blood for changes in expression patterns of mRNAs and miRNAs related to the histaminergic system?
Q2. How does this dual approach contribute to our understanding of the molecular landscape in women with endometrioid endometrial cancer?

Dear author thanks in advance for your kind support answering the questions.

Now, the conference publication phase is close but you can answer here directly as a post-publication comment.

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