Nanobiotechnology is a broad science that provides nanomaterials to solve many medical, agricultural, engineering, biological and chemical problems. Metallic nanoparticles are the kind of nanomaterials with zero dimensions. Nowadays, the synthesis of semiconductor nanoparticles has received much attention due to its many applications in various scientific fields. In general, nanoparticles can be synthesized by three physical, chemical and biological methods. In this study, ZnO-NPs were synthesized using biological method. In the biological method, the aqueous extract of Juglans regia green husk was used as an agricultural waste material to produce nanoparticles. Therefore, in this research, nanoparticles were synthesized by the green method and their chemical and physical properties were determined. The properties of the synthesized nanoparticles were investigated by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and transmission electron microscopy (TEM). The results of UV-Vis analysis showed that the biosynthesized zinc oxide nanoparticles have a strong SPR band at 360 nm. XRD analysis showed that these nanoparticles are crystalline in nature and FTIR revealed the capping agent of these nanoparticles. Also, the results of DLS analysis showed that the hydrodynamic diameter of 90% of the biosynthesized zinc oxide is less than 292.55 nm. Furthermore, TEM analysis confirmed that these nanoparticles have spherical and ellipsoidal shapes.