Cystitis is an inflammatory condition that primarily affects the bladder. It is often caused by a bacterial infection, with bacterial cystitis being the most common type among various urinary tract infections. This research aimed to extensively document ethnobotanical knowledge regarding the use of medicinal plants for treating cystitis due to their proven therapeutic properties. The study was conducted in the Rif region from March 1st, 2020, to April 15th, 2020. Semi-structured direct interviews were conducted with 657 participants to gather indigenous therapeutic wisdom. These surveys included information about the interviewees' demographics and ethnomedicinal practices. UR and MUV techniques were employed in data analysis. A total of 60 plant species, distributed among 51 genera and 31 families, were commonly used by our interviewees for cystitis therapy. Apiaceae had the highest representation of seven species, and Capparis spinosa L. was the most frequently recommended medicinal plant by the local population. Leaves were the most commonly utilized plant part (41.5%), and most herbal remedies were prepared through decoction (55%). This study constitutes the initial contribution to the ethnobotanical exploration of this region. It is recommended that the natural plant species identified in this research be further investigated to uncover their therapeutic effects and mechanisms of action. Primary attention should be given to conserving medicinal species, thoroughly documenting widespread medicinal knowledge, and biologically validating the listed species.