Design of an electrochemical genosensor for the BDNF gene polymorphism sequence detection using an enzymatic labelled DNA probe

Renato Caldevilla; Stephanie Morais; Serafim Carvalho; Rui Medeiros; Cristina Delerue-Matos; Agostinho Cruz; Marlene Santos; M. Barroso

doi:10.3390/CSAC2023-14913

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Design of an electrochemical genosensor for the BDNF gene polymorphism sequence detection using an enzymatic labelled DNA probe

^{1, 2},

²,

^{3, 4},

^{5, 6},

Cristina Delerue-Matos

²,

Agostinho Cruz

¹,

Marlene Santos

^{*

1, 5},

M. Fátima Barroso

^{*

2}

¹ CISA|ESS, Centro de Investigação em Saúde e Ambiente, Escola Superior de Saúde, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 400, 4200-072, Porto, Portugal
² REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico, Rua Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal
³ Hospital de Magalhães Lemos, 4149-003 Porto, Portugal
⁴ Instituto Universitário de Ciências da Saúde, 4585-116 Gandra, Portugal
⁵ Molecular Oncology & Viral Pathology, IPO-Porto Research Center (CI-IPOP), Portuguese Institute of Oncology, 4200-072 Porto, Portugal
⁶ Research Department, Portuguese League Against Cancer (Norte), 4200-172 Porto, Portugal

Academic Editor: Nicole Jaffrezic-Renault

Published: 26 September 2023 by MDPI in The 2nd International Electronic Conference on Chemical Sensors and Analytical Chemistry session Electrochemical Devices and Sensors

https://doi.org/10.3390/CSAC2023-14913

Abstract:

The BDNF gene is associated with high degrees of variability in antidepressant treatments. The Val66Met polymorphism is widely known as a source of this variability, warranting growing interest in genotyping patients that undergo antidepressant treatment to better suit their needs. This paper reports on an electrochemical genosensing platform, based on gold electrodes, capable of detecting this polymorphism, through the use of synthetic enzymatic labelled DNA-probes for 2 different BDNF alleles. The sensor showed promising results, and its applicability to real samples is currently being tested.

Keywords: Chronoamperometry; Depression; Electrochemical genosensor

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