Laser-induced breakdown spectroscopy (LIBS) was explored to assess the mineral constituents (Ca, Mg and N) in the skin, pulp, and seed of two Vitis vinifera cultivars - a white (Loureiro) and a red (Vinhão) cultivar. This study compares the two grape cultivars chosen and the characterisation of Ca, Mg and N in the skin, pulp and seed on three dates after veraison. Significant differences (p< 0.05) were found in the Ca, Mg and N in the skin, pulp and seed of both grape cultivars during the three assessment dates considered. The results of this study could provide insights into the mineral composition of grapes, offering a fast, accurate, and cost-effective alternative to traditional mineral quantification methods.