The search for innovative therapeutic solutions to address the escalating resistance of pathogens to antibiotics is a pressing global challenge. Bioprospecting for novel molecules is a cornerstone, providing essential data to fuel databases used in developing AI-driven tools. These tools are crucial for drug design, ushering in a new drug discovery and development era. Antimicrobial peptides (AMPs) have demonstrated their effectiveness against a broad spectrum of pathogens. These peptides are sourced from various natural origins, including amphibian skin, which is particularly abundant. The northern region of Argentina boasts an unexplored and diverse amphibian biodiversity, promising potential for the discovery of new AMPs. This study aimed to identify AMPs from Leptodactylus chaquensis frog skin collected in Corrientes, Argentina. Total mRNA was extracted from the skin, cDNA was synthesized, and cloned into E. coli DH5α cells. Plasmids of selected colonies were purified, PCR amplified, insert size assessed, and sequences obtained. Three prepro-peptides coding novel mature peptides were identified. The peptides, provisionally named Lch-1, Lch-2, and Lch-3, exhibited lengths of 25, 21, and 9 residues, with net charges of +1, 0, and -1, respectively. Hydrophobic percentages were 38%, 54%, and 44%, with theoretical 3D α-helical content in their structures. Lch-1 and -2 shared 43% and 41% sequence similarity with ocellatin-4 and -6 from Leptodactyus ocellatus. Predicted interactions with membranes suggest antimicrobial potential using the APD tool. Peptides will be synthesized for bioactivity assays. These findings underscore the significance of bioprospecting in uncovering distinct and unique bioactive compounds within each species.