The increased concentration of pollutants is a challenge for the population health. This work aims to investigate the health risk that is related to pollutants’ level in the center of Rhodes city. Rhodes Island is a desirable tourist destination with important economic activity over the southeastern Aegean Sea. The analysis covers the (summer) July-August months and the (winter) December month of 2022. Hourly recordings of the concentrations of PM2.5, NO2 and O3 from a mobile Air Quality Monitoring System (AQMS) are analyzed. In order to investigate the effects of pollution level on people’s health, the Air Quality Health Index (AQHI) is calculated. Results show that summer compared to winter period shows increased health danger possibly due to the increased traffic emissions, tourist density as well as the different meteorological conditions. In summer period, the AQHI is classified between the upper Low to Medium health risk class and during winter month, AQHI is classified in Low health risk class, respectively. The AQHI diurnal variability is higher during July-August compared to December. Additionally, the diurnal differences between two periods show increased health risk for summer months mainly between the hours from early evening to early morning. Finally, the analysis shows that the traffic activities affect the health risk and highlight that the authorities should adopt green policies to protect human’s health.