Urticaria it is a autoimmune disease and many patients are suffer from it. The research aims to investigate the development and characterization of Ebastine-loaded transfersomal nanogel for enhancement of bioavailability in the treatment of urticaria. The flexible transfersomes, consisting of the drug Ebastine, soya lecithin, and edge activator Tween 80, were prepared with the thin film hydration method. The transfersomal nanogel was formulated by using the dispersion method and utilizing a suitable concentration of the gelling agent Carbopol 934. Transfersomes and its gel were evaluated for various parameters. The Ebastine loaded transfersome showed the highest entrapment efficiency, up to 79.92%. The polydispersity index (PDI) of the transfersomes was determined to be 0.103 and the zeta potential to be -18.9 mV, indicating that the formulation was stable. The drug content of the transfersome gel was found to be 83.67%. Transfersomal gel formed using 1% Carbopol 934 showed the best results, showing in-vitro release up to 8 hours and following a zero-order kinetic model. As per the microbial studies conducted, the Ebastine transfersomal gel has a good anti-microbial impact against S. aureus. These vesicular transfersomes are more flexible than other vesicular systems, making them excellent for skin penetration. In future it will be a best possible approach for the delivery of drug via transdermal route.