The present study aims to comprehensively assess the antioxidant, anti-inflammatory, anti-diabetic, and anti-cancer potential of hydro-methanolic extracts derived from dried noni fruits and seeds grown in Sri Lanka. The extraction process involved mixing one gram of fresh fruit flesh samples with 8 mL of 80% methanol, followed by vortexing and centrifugation. The resulting extracts underwent filtration and were subsequently utilized in multiple colorimetric assays. The IC50 values of total antioxidant capacity for dried noni fruits and seeds were found to be 38.17 ± 1.23 µg/ml and 39.79 ± 0.30 µg/ml, respectively. Moreover, the IC50 values of alpha-amylase inhibitory activity for dried noni fruits and seeds were 22.62 ± 0.46 µg/ml and 19.70 ± 0.56 µg/ml, respectively. Additionally, the IC50 values of proteinase inhibitory activity for dried Noni fruits and seeds were observed to be 26.28 ± 0.22 µg/ml and 19.31 ± 0.21 µg/ml, respectively. Further analysis using the MTT assay revealed the median lethal concentrations (LC50) of the dried crude extracts of Noni fruits and seeds on the cytotoxicity of BHK normal cells to be 1.0409 µg/ml and 1.6822 µg/ml, respectively. For Hep2 cancer cells, the LC50 values were 0.9537 µg/ml and 1.08 µg/ml, respectively. These findings suggest that the hydro-methanolic extracts of dried Noni fruits and seeds exhibit promising bioactivity, making them potentially valuable candidates for further investigation and the development of natural remedies for various health conditions, including cancer.