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Incorporation of Actinidia arguta extract in mucoadhesive films – A new oral mucositis prevention/treatment approach
* 1 , 1 , 1 , 2, 3 , * 1
1  REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto
2  REQUIMTE/UCIBIO, MedTech-Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto
3  Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto
Academic Editor: Marco Annunziata


Actinidia arguta is a perennial vine that mostly grows in Asian countries, being described as a traditional herbal medicine in Korea [1]. The fruit, commonly known as kiwiberry, has been associated with different therapeutic properties and pro-healthy benefits for consumers, particularly antioxidant, anti-inflammatory and anticancer effects [2-4]. These bioactive properties are due to the outstanding content in phenolic compounds, vitamins, and organic acids [4], which attracted the researcher’s attention for potential application in nutraceutical and pharmaceutical industries. This is the case of oral mucositis (OM), a common side effect of cancer treatments that causes oral inflammation and pain [5]. OM can take advantageous of these natural bioactive compounds to alleviate symptoms and promote healing [6, 7]. Therefore, the aim of this work is to formulate mucoadhesive films to prevent/treat OM symptoms through incorporation of A. arguta extract. The films were prepared by solvent casting method employing 1% of HPMC K100 LV EP solution with 2.5% glycerin, and A. arguta extract as solvent. Different parameters were assessed on films, namely physical features (weight: 194.8 mg; thickness: 0.37 mm; disintegration time: 15.05 min; superficial pH: 4.20; moisture content: 10.53%; swelling capacity: 55.95 %) as well as mechanical properties (resistance to extension: 10.11 N; percent elongation: 33.64%; Young’s modulus: 0.0034 MPa). The in vitro cell assays revealed that films did not affect the viability of HSC-3 and TR146 oral cell lines. Additionally, the compounds release profile is being performed by in vitro and ex vivo permeation assays coupled to LC-MS quantification.

This work received financial support from project EXPL/BAA-GR/0663/2021 – Kiwi4Health – Exploring the Eco-Innovative Re-Use of Kiwiberry, supported by national funds by FCT/MCTES and by the projects UIDB/50006/2020 and UIDP/50006/2020 through national funds. This work was also financed by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020, UIDB/04378/2020 and the project LA/P/0140/2020.Filipa Teixeira is thankful for the scholarship from project EXPL/BAA-GR/0663/2021. Ana Margarida Silva is thankful for the Ph.D. grant (SFRH/BD/144994/2019) financed by POPH-QREN and subsidized by the European Science Foundation and Ministério da Ciência, Tecnologia e Ensino Superior. Francisca Rodrigues (CEECIND/01886/2020) is thankful for contract financed by FCT/MCTES—CEEC Individual Program Contract.

[1] Kwon, D., et al., Pinoresinol diglucoside is screened as a putative α-glucosidase inhibiting compound in Actinidia arguta leaves. Journal of the Korean Society for Applied Biological Chemistry, 2014. 57(4): p. 473-479.
[2] Silva, A.M., et al., Influence of temperature on the subcritical water extraction of Actinidia arguta leaves: A screening of pro-healthy compounds. Sustainable Chemistry and Pharmacy, 2022. 25: p. 100593.
[3] Zhang, J., et al., Phenolics Profile and Antioxidant Activity Analysis of Kiwi Berry (Actinidia arguta) Flesh and Peel Extracts From Four Regions in China. Frontiers in Plant Science, 2021. 12.
[4] Macedo, C., et al., Insights into the polyphenols extraction from Actinidia arguta fruit (kiwiberry): A source of pro-healthy compounds. Scientia Horticulturae, 2023. 313: p. 111910.
[5] Ferreira, A.S., et al., Natural Products for the Prevention and Treatment of Oral Mucositis—A Review. International Journal of Molecular Sciences, 2022. 23(8): p. 4385.
[6] Landová, H., et al., Evaluation of the Influence of Formulation and Process Variables on Mechanical Properties of Oral Mucoadhesive Films Using Multivariate Data Analysis. BioMed Research International, 2014. 2014: p. 179568.
[7] Nair, A.B., et al., In vitro techniques to evaluate buccal films. Journal of Controlled Release, 2013. 166(1): p. 10-21.

Keywords: Actinidia arguta; oral mucositis; mucoadhesive films; solvent casting