Blueberries are popular all over the world due to their flavorsome fruit, abundance of bioactive compounds, and antioxidant activity. However, many countries discard large quantities of leaves, so exploiting the bioactive compounds in the leaves would be very beneficial for the agricultural industry. Studies on these residues that identify phytochemicals and evaluate their biological effects have recently been conducted in our group (ref)Therefore, this study highlights the applications of blueberry leaves, focusing on their dermatological, antioxidant, antimicrobial, and antibiofilm activities. The aim was to assess the feasibility of using this by-product in the prevention and treatment of skin problems its antimicrobial efficacy against relevant skin pathogens and its ability to prevent biofilm formation. The results reveal remarkable properties: a total antioxidant activity of 84.21%±0.1, a complete inhibition of hyaluronidase (100%), 55.3 ± 4.2% attenuation of elastase, and 61.2 ± 10.5% suppression of tyrosinase. The antibiofilm results also show substantial inhibitory effects, with a mass quantification inhibition rate of 38% against Cutibacterium acnes and 33% against Staphylococcus aureus, accompanied by a 34% and 48% reversal of their respective metabolic activities. The results obtained indicate that blueberry leaves could be an interesting by-product to be applied as a component of cosmetic and dermatological preparations with antioxidant, antimicrobial, and anti-biofilm properties