Levodopa, or L-3,4-dihydroxyphenylalanine, is an amino acid directly obtained from phenylalanine and a medicine widely used for the effective treatment of Parkinson's disease. The main aim of this work was to clearly highlight and develop two quantitative synthesis methods of two new azo dyes possessing chromophore groups, which were obtained by the color reactions of Levodopa with two main chromogenic agents—alpha-naphthol 0,1% and beta-naphthol 0,1 % alcoholic alkalinized solutions—in the presence of NaNO₂ 5% and HCl 15%-20%. Following the first color reaction of Levodopa with alpha-naphthol, an intensely bright yellow azo dye was quantitatively obtained and showed a maximum absorption at λ = 418 nm. In the second case, as a result of the second quantitative reaction of Levodopa with beta-naphthol, an intense orange azo dye with a reddish tint at λ = 478 nm was synthesized. Both azo dyes quantitatively obtained from Levodopa had the azo- -N=N- reactive groups as color-generating chromophores directly assigned to the signaling moieties of two chemical sensors. In the first reaction, the azo group was directly linked to alpha-naphthol, and in the second reaction, the azo group was linked to the beta-naphthol aromatic cycle. An important objective of this research was to accurately quantitatively analyze Levodopa in a pharmaceutical following these two new color reactions of L-Dopa with alpha-naphthol 0,1% and beta-naphthol 0,1 %. According to these reactions, two new azo dyes were quantitatively obtained from Levodopa and could be spectrophotometrically dosed at λ = 418 nm for the intensely bright yellow azo dye and λ = 478 nm for the intense orange azo dye with a reddish tint. Through the spectrophotometric quantitative analysis of the two azo dyes formed at λ = 418 nm and λ = 478 nm, it was possible to successfully and accurately quantify Levodopa in a pharmaceutical.