Biofilm formation is a bacterial phenotype by which bacteria can make antibiotic resistance. The extracellular polymeric substances (EPS) matrix of biofilm protects bacteria against environmental stresses, including antibiotics, immunological reactions, and disinfectants. The inhibition of antibiotic resistance is now a concern in medical science. In nanotechnology area nanoparticles are showing promising effect in inhibiting biofilm formation. Pseudomonas aeruginosa, Streptococcus pneumoniae, Listeria monocytogenes, and Escherichia coli are some of the bacteria that are susceptible to the powerful antibacterial and antibiofilm activities that are exhibited by several nanoparticles. These nanoparticles can generate reactive oxygen species (ROS) and release ions, which make them effective for inhibiting biofilm formation. Some nanoparticles bind with bacterial membrane or bacterial protein to remove antibiotic resistance and to make them possible in antibiofilm activities. The effect of nanoparticles in antibiofilm activity may depend on size. Because generating reactive oxygen species (ROS) and releasing ions property is dependent on size. This statistical study will show the utilization of nanoparticles in inhibiting antibiotic resistance by inhibiting quorum sensing and by inhibiting biofilm formation will depend on their size. We will demonstrate the effect that the sizes of the NPs have on the antibiofilm activity through our investigation. In this instance, we obtained information needed for data analysis from a variety of research articles. The descriptive analysis and cross-sectional study are all components of our analysis, which involves employing those data. The results of this investigation will indicate that size influences the antibiofilm activity.