Public concerns over animal welfare have increased scrutiny of the early separation of calves from cows. Overall, women are generally more supportive of practices allowing prolonged cow–calf contact, potentially due to their greater empathy in terms of animal welfare. This study examines how gender affects Brazilian livestock advisors' attitudes toward promoting dairy welfare practices, particularly cow–calf contact systems. By analyzing gender-based differences, this research highlights how advisors’ perspectives may influence the adoption of welfare practices aligned with public expectations. Hence, the aim of this study was to assess the effect of gender on Brazilian livestock advisors’ attitudes towards their role in promoting dairy cattle welfare. Through an online questionnaire featuring a three-point Likert scale, we investigated topics related to general dairy cattle welfare and the role of advisors in mediating between societal demands and dairy production practices. The effect of gender on the Likert-scale responses was analyzed using an Ordinal Regression Analysis. The results from the Likert scale are presented as the percentages of participants for each Likert-scale option and the predicted mean values ± standard deviation. The participants (n=176) were predominantly male (59%) and aged between 18 and 35 years (58%). In general, 81% of the participants acknowledged the influence of public pressure on dairy farming practices (Likert 2.74±0.5), and the majority (71%) stated that farmers are consistently provided with information on animal welfare practices (Likert 2.65±0.6). Almost all of the participants (91%) felt fully responsible for helping to address animal welfare issues (Likert 2.89±0.4). There was a trend (p=0.06) of men self-reporting a higher knowledge (Likert 2.76±0.6) of animal welfare compared to that in women (Likert 2.6±0.7). Conversely, the women showed more positive attitudes (Likert 2.54±0.7; p=0.009) than men (Likert 2.22±0.8) toward discussing systems that allow cow–calf contact. In conclusion, while men tend to perceive themselves as more knowledgeable about animal welfare, they are less inclined to engage in discussions about cow–calf contact.