The potential zoonotic transmission route of Enterocytozoon bieneusi and Encephalitozoon spp. (E. intestinalis, E. hellem and E. cuniculi) is under discussion. Following the detection of human-related microsporidia in dog faecal samples collected in Leicester city centre (UK) in January 2016, we monitored the presence of human-related microsporidia in 628 fresh pigeon faecal samples collected in urban and suburban areas of Leicester between summer 2017 and summer 2018, as this invasive species closely interacts with humans, especially with susceptible individuals such as children or the elderly. The presence of microsporidian spores was assessed using Weber's chromotrope stain. Microsporidian species were detected in all samples by SYBR Green real-time PCR with melting curve analysis after DNA extraction by spore disruption using Fast-Prep for soil^® following previous methodologies. Spores were observed in 104 samples, with 81 testing positive by molecular methods. Specifically, E. bieneusi was detected in 13 samples, while 26, 17 and 9 samples tested positive for E. intestinalis, E. hellem and E. cuniculi, respectively. Moreover, 16 samples were positive for E. hellem/intestinalis. Despite being the species with clearer zoonotic implications, the lower presence of E. cuniculi in the pigeons could be logical, as this species has not been found in similar studies, e.g., in pigeons monitored in public parks in Madrid, Spain. Although our results suggest a moderate presence of these microsporidian species in pigeons in Leicester, the prevalence is slightly lower than that reported in the literature. However, the prevalence found may represent a potential risk to residents and visitors to the city of Leicester and its surrounding areas, particularly when they flap their wings, as this movement may facilitate the resuspension of their faeces with debris and other topsoil materials. Further studies monitoring these parasites in the intestines of pigeons are needed to properly characterise the risks identified.