In our research work, we used type-Y chicken antibodies in treatment and prevention of pasteurellosis in horses. Currently, our knowledge and experience of chicken antibodies use to fight variety of human and animal disease of different ethologies is growing. In September 2022, five Karabakh horses were transformed to a zoo in Baku from Horse factory located 320 km away. One horse died on the third day after the arrival. Bacterial investigation of parenchymal organs indicated Pasteurella multocida. As a preventing treatment for other horses, we used an intramuscular injection medicament of immunoglobulins IgY, extracted from fowl egg yolk, hyperimmunised with Pastanarm-8 containing antigen Pasteurellosis. The purpose of the research is to study the use of type-Y immunoglobulins, extracted from chicken egg yolk, hyperimmunised with a vaccine against pasteurellosis of agricultural animals.
1 yo chickens are hyperimmunised with Pastanarm-8 vaccine, containing eight antigens of various infection triggers, Pasteurellosis among them. After extraction of the immunoglobulins by deposition with ammonium sulphate and then distilled water dialysis, by Bradford method, we found that in 1ml solution there are 50 mg of immunoglobulins. The solution is intramuscularly injected to each horse in a doze of 160 ml.
Daily thermometry and clinical tracking of the horses showed that deviation from the norm started to disappear from the second day. However, problems were discovered in the neighbouring paddock, containing sheep and goat, that were served by the same personal. Two sheep died. Clinical and laboratory research shoed that pastorales was the cause. Methodic action to liquidate Pasteurellosis among the zoo animals were taken immediately. After 14 days, an all inclusive Pastanarm-8 vaccination was conducted. Pasteurellosis epizootic among the zoo animals was eliminated. Successful use of chicken immunoglobulins in prevention of Pasteurellosis indicates that it is possible to use it as an alternative to horse serum, which has several negative features.