Under proper conditions, green lacewings (Neuroptera: Chrysopidae) larvae show great voracious predatory habits, being an effective biological control species against several pest insects. They are constantly exposed to insecticides as much as their prey on the field, which could impair their lifespan and reproduction, and so far, there are no molecular data available showing the impact of the insecticide pyriproxyfen on testes of Ceraeochrysa claveri. Even when presenting some cellular damage and ultrastructural alterations in the testes cells, this species had already demonstrated tolerance against the insecticide pyriproxyfen, being able to reproduce and maintain their lifespan under lab conditions. Being of great ultrastructural importance, Talin is a protein that connects to the cytoskeleton by the actin and vinculin binding sites and enhances the integrin connection to the extracellular matrix, maintaining the cell structure. This work aimed to evaluate the expression of Talin on testes of C. claveri adults treated with pyriproxyfen during the larval phase by RNA-seq. Newly hatched larvae (0-12h) of C. claveri were individualized, fed on Diatraea saccharalis eggs treated at two concentrations of pyriproxifen (50 mg and 100 mg a.i. L^-1), and compared to a control group (no insecticide intake) for 10 days. After that, all insects fed on eggs with no insecticide until pupation. When adults emerged, 10-day-old males were dissected, with the testes removed and taken for RNA extraction, library construction, and sequencing. The reads were de novo assembled and the genes were annotated by scanning the ORFs for homology. Differentially expressed genes (DEGs) were observed among the groups (50mg vs control; 100mg vs control; and 50mg vs 100mg). RNA-seq results showed that Talin was upregulated in the 50mg vs control and 100mg vs control groups, with both results confirmed by RT-qPCR. These molecular results could help improve Integrated Pest Management (IPM) strategies to better preserve green lacewings on field.