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Characterization and evaluation of formulations based on shrimp co-products for the protection of stored semolina.
* 1 , 2 , 1
1  National Agricultural Research Institute of Tunisia (INRAT), Laboratory of Biotechnology Applied to Agriculture, University of Carthage, LR11INRAT06, 1004, Rue Hedi Karray, El-Menzah, Tunis, Tunisia.
2  FST
Academic Editor: Louis Hesler

Abstract:

This work focused on the evaluation of insecticidal activity by the fumigation of essential oils of Laurus nobilis and Thymus vulgaris and the formulations encapsulated in a powder matrix obtained from shrimp and chitosan against adult Rhyzopertha dominica. Chromatographic analyses identified 21 and 16 compounds, respectively, for T. vulgaris and L. nobilis essential oils, including 1,8-cineole (43.23 %), α-Terpinene (17.47 % ) and β-Linalool (14.86 %) for L. nobilis, and Carvacrol (75.01 %) and o-cymene (7.93 %) for T. vulgaris. The fumigant toxicity of crude essential oils depended on the concentration and storage period. Indeed, after 10 days of storage, LC50 values were 73.19 and 81.92 µL/ L air,respectively, for L. nobilis and T. vulgaris compared to59.85 and 64.66 µL/ L, respectively, after 20 days storage. The characterization of shrimp co-products revealed that the powder contains essential protein (21.24 %) and fats (17.05 %). In addition, these co-products exhibited 10.03 % of moisture content and 26.67 % of ash content. The insecticidal activity of L. nobilis and T. vulgaris essential oil formulations encapsulated in shrimp powder increased during the storage period. The toxicity of the formulations increased significantly with increasing concentration and storage period. Indeed, after 10 days of storage, LC50 values varied from 12.03 to 29.88 g/ L air, respectively, for L. nobilis and T. vulgaris compared to3.65 and 7.78 g/ L air after 30 days of storage. In addition, the formulations decreased the number of emerged larvae. The results of the present study highlighted the promising potential of the formulations prepared from the powder of shrimp co-products for the control of insect pests of stored food.

Keywords: Shrimp co-products; Laurus nobilis; formulation; Rhyzopertha dominica; semolina; Thymus vulgaris
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