In recent decades, the use of animals in research has been reduced due to the requirements of ethics committees and good laboratory practices. However, total replacement is not yet feasible, as animals are essential for studies that seek to understand human physiology. Insects such as Galleria mellonella have been used as infection models, presenting advantages such as easy reproduction, incubation between 25 and 37 °C, and phagocytic cells called hemocytes, similar to mammalian phagocytes. G. mellonella larvae have six types of hemocytes, essential in the immune system, with variable density in the hemolymph, which can be measured to evaluate responses to infections. The viability of the larvae is verified by the absence of movement and intense melanization, indicators of response to pathogens. Thus, G. mellonella has been used to evaluate the virulence of pathogens and the toxicity of substances. In the test performed, groups of 10 larvae (200–250 mg) were subjected to toxicity analyses. After 24 hours without feeding at 37 °C in the dark, the larvae had their prolegs sanitized with 70% ethanol and were injected with 10 μL of extracts (2500 μg/mL), fractions (2500 μg/mL), or solvent (DMSO 2.5%) using a glass syringe previously cleaned with hypochlorite, ethanol, and saline solution. The larvae were kept at 37 °C and monitored at intervals of 2 to 24 hours, and then daily for seven days. The results indicated a natural mortality of up to 10% in the control group, while all extracts showed a mortality below 20%.