Abstract: Background: Antifungal resistance is gradually becoming a much more frequent problem. The limited number of available drugs and growing resistance especially as it is a common infection with pregnant women mostly caused by Candida albicans has become a serious issue worldwide. Previous studies have shown that approximately 70-75% of women will have at least one episode of vulvovaginal candidiasis with half of that percentage having recurrent infection. Objective: The aim of this study was to determine the antifungal resistance profile of yeast involved in vulvovaginal candidiasis infections in pregnant women in the Buea Regional Hospital. Method: This cross-sectional study was carried out from the 20th of May to the 30th of June. The volunteers were given questionnaires in order to obtain their socio-demographic data and vaginal swab samples. The samples were cultured on Sabouraud Dextrose Agar, gram stain microscopy was done and susceptibility tests carried out to identify Candida strains. The data was recorded in Microsoft Excel 2010 and analyzed using SPSS version 20. Results: A total number of 92 participants took part in this research of varying ages with the highest percentage of 48(52.2%) being women within the age range 26-36 years. The prevalence was 51(55.4%) for positive cases and 41(44.6%) for negative cases. The prevalence of the species determined from the positive cases were 40(78.4%) for C. albicans and 11(21.6%) were non-albicans candida species. The sensitivity differed with the species that is, C. albicans: KCA-10 21(52.5%), and FLC-25 12(30%). None of the drugs were sensitive for non-albicans candida species (NAC). Conclusion: The limited antifungal drugs are growing highly resistant to yeast infections especially for NAC and the rate of infection amongst pregnant women remain quite high.