Introduction: Endometriosis is a chronic gynecological disorder affecting approximately 7–10% of menstruating individuals worldwide. Despite its high prevalence, the etiopathogenesis of endometriosis remains largely unclear. Emerging evidence suggests that environmental contaminants such as cadmium (Cd), nickel (Ni), and lead (Pb) may contribute to its development.
Methods: Peritoneal fluid (PF) samples were obtained from 50 subjects (25 controls and 25 patients with endometriosis) attending the Gynecology Service of San Juan University Hospital in Spain between June 2020 and September 2022. Samples were processed and analyzed by inductively coupled plasma tandem mass spectrometry (Agilent 8900 ICP-MS/MS). Statistical analyses were conducted using IBM SPSS Statistics version 28.0 (IBM, Armonk, NY, USA) and Bayesian Kernel Machine Regression (BKMR) was performed in R (version 4.4.3, package “bkmr” and “ggplot2”) to provide new insights into the effects of Cd, Pb, and Ni on endometriosis risk.
Results: Cadmium levels were significantly higher in the endometriosis group compared to the control group (0.71 ± 0.94 vs. 0.25 ± 0.22 µg/L, p=0.009). The BKMR model using PF concentrations of Cd, Ni, and Pb revealed a positive trend in endometriosis risk with increasing metal exposure. However, this combined effect was not statistically significant, and none of the metals showed an independent association with the disease.
Conclusions: Although no statistically significant associations were observed, the positive trend in the combined effect of Cd, Ni, and Pb suggests a potential role of metal mixtures in endometriosis pathogenesis. This lack of statistical significance may be related to sample size limitations and inherent variability in peritoneal fluid composition. Nevertheless, the observed trend is consistent with the previous literature on the endocrine-disrupting properties of these metals. These findings highlight the importance of considering environmental mixtures in research on reproductive health and gynecological diseases, and emphasize the need for larger studies to better understand these associations.
