An investigation into the antimicrobial effectiveness of gold nanoparticles and lasers against various bacterial strains.
As a catalyst, AuNPs are one of many NPs that have found application in gene therapy, medical therapy, and biological diagnostic methods, as well as NPs used in gene therapy. An in vitro study was conducted to assess the effectiveness of gold nanoparticles (AuNPs) and gold nanoparticle–low-level-laser-combined therapy against bacterial isolates. The bacterial growth rate and medium inhibitory concentrations of AuNPs were determined at five concentrations (25, 50, 75, 100, and 200 g/mL). After treatment with gold nanoparticles, bacterial growth was found to be slightly reduced. Also, gold nanoparticles were tested for their influence on Staphylococcus aureus biofilms. The amount of biofilm that can be formed was reduced by different concentrations of AuNPs when compared to a control without nanoparticles. A combination of low-level lasers and AuNPs produced an improved cytotoxic effect against Escherichia coli and Staphylococcus aureus, indicating that the combination of low-level lasers enhanced AuNPs' antibacterial activity. As a result of this work, we were able to explore the effectiveness of gold nanoparticles and the AuNP-laser-induced therapeutic approach (at different energy densities of lasers and different concentrations of AuNPs) as antibacterial agents against some pathogenic bacterial strains with gold nanoparticles.
Keywords: Gold nanoparticles, Laser, Antibacterial agent, Cytotoxicity, MIC.