A controlled study was conducted on the susceptible olive cultivar "Picholine Marocaine" to examine the relationship between Verticillium dahliae and Rhizophagus irregularis. After three months of pre-inoculation with R. irregularis, olive trees were post-inoculated with V. dahliae for nine months. The current study evaluated how combined inoculation affected disease tolerance, nutrient uptake, root colonization, and plant development factors. The findings demonstrated that, even when V. dahliae was present, R. irregularis considerably enhanced mycorrhizal colonization, including the development of vesicles and arbuscules, in comparison to non-mycorrhizal controls. Increased shoot and root lengths, more leaves and branches, and a larger total dry biomass were all indicators of better plant growth that was linked to enhanced colonization. Lower dwarfing and leaf alteration indices further demonstrated that R. irregularis considerably lessened the severity of the disease. Additionally, mycorrhizal inoculation significantly increased the uptake of nutrients, especially potassium, calcium, and available phosphorus, in the roots and shoots of olive plants. Remarkably, plants colonized exclusively by R. irregularis had increased salt uptake, indicating a possible influence on particular nutrient dynamics. Overall, this study shows that R. irregularis greatly increases disease tolerance against Verticillium wilt in sensitive olive cultivars, boosts plant growth and nutrient uptake, and efficiently encourages root colonization.