Introduction. This paper examines current aspects of the development and medical introduction of new disinfectants based on heterogeneous surface matrices exhibiting microbicidal activity, in the context of increasing resistance to existing drugs. A comprehensive approach is proposed for characterizing the properties of the synthesized matrix samples using both pharmacopoeial techniques and original methods . The purpose of this study was to develop, synthesize, and analyze materials with surface antimicrobial properties based on metallic silver nanoparticles (AgNPs). The study objects included: A nanodispersion containing metallic silver particles (AgNPs) synthesized by the citrate method using a 2 mM AgNO₃ solution (pH 5.4), 0.15 M sodium citrate, and 0.15 M glucose at 60°C; The formation of AgNPs was monitored by UV-Vis spectroscopy by sampling aliquots every 5 minutes during synthesis; A heterogeneous solid medical Paraffinum served as a model material with . It was prepared by adding an AgNPs suspension to molten paraffin (approximately 0.5 cm layer), continuously heated by a horizontal element, using spraying under mixing mimicking vortex water-oil emulsification. After synthesis, the nanostructured heterogeneous paraffin (NGP) sample was left to solidify completely. To assess the quality of both the nanodispersion and NGP, the following methods were applied: Dynamic Light Scattering (DLS, Zetasizer Nano ZS), UV-Vis spectroscopy (Agilent Cary 300), and surface diffuse reflection analysis. Results. The AgNPs in the nanodispersion exhibited an average particle size of approximately 150 nm (intensity distribution). The UV absorption peak at 440 nm corresponds to surface plasmon resonance and 4d‒5sp interband electronic transitions. Chemometric analysis of speckle patterns formed by diffuse reflection from the heterogeneous surface provided a unique descriptor distribution enabling qualitative identification. Conclusions. he results may be valuable for the pharmaceutical industry in the synthesis, quality control, and application of new bactericidal systems. Funding: This research was supported by a grant from the RUDN University Strategic Academic Leadership Programme No. 033320–2-000.