Abstract: In this study, nonionic surfactants were extracted from ginseng. The extraction of these surfactants from the plant was done by soxhlet. After the extraction, we used FT-IR spectroscopy for characterization of extracted saponin. Rates of change of water surface tension in terms of these surfactants concentration were obtained at 25°C. It shows that saponin as a surfactant decreased the surface tension of water. After that, the critical micelle concentration of these surfactants was obtained by curve of change of water surface tension in terms of change of these surfactants concentration.