Large-scale biomass production of Saccharomyces cerevisiae is essential for oenological and industrial applications. The efficiency and cost-effectiveness of biomass cultivation depend significantly on the composition of the culture medium. In this study the potential of different nitrogen sources for yeast growth was evaluated in different medium cultures, evaluating the growth of four different commercial S. cerevisiae. The different nitrogen sources used for the culture medium were easily available in the cellar as brewer’s yeast, diammonium phosphate and two commercial products with nitrogen composition, Actimax Vit and Actimax Plus; all of them were tested in different concentrations. Fermentations with different culture mediums were performed on a 96-well plate at 24ºC with no oxygenation or extra feeding. Cell growth was evaluated at initial and final stages by solid culture and monitored by OD₆₀₀ by microplate reader Varioskan lux. The medium containing Actimax Plus did not support satisfactory yeast growth, despite having a composition similar to the commercial medium. Additionally, brewer’s yeast-based media were discarded due to their complex handling under cellar conditions. The medium with sucrose and Actimax Vit and diammonium phosphate maintained yeast growth yields and represented a more cost-efficient and promising alternative for biomass production compared to the commercial medium evaluated.