Medicinal plants are precious reservoirs of bioactive molecules; they are able to produce diverse natural substances. Currently, research is focused on creating new applications and taking advantage of the qualities of these resources in many industries, including the food, cosmetics, and pharmaceutical industries [1]. In line with this global interest in medicinal plants In this work we explore the antimicrobial properties of the Eriobotrya japonica, the extract has been obtained after plant leaves were meticulously collected during their optimal growth period, followed by a careful process of drying and grinding. Employing the Maceration technique, we utilized ethanol as the solvent for extraction, with subsequent defatting accomplished using hexane. The total phenolic content in the Eriobotrya japonica extract was determined to be 384.19 µg/mL gallic acid equivalents, indicating that the plant is rich in phenolic compounds. The antioxidant activity also was evaluated by various antioxidant methods, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azinobis (3-ethylbenzothiazolin) -6-sulphonic acid (ABTS), Ferric Reducing Antioxidant Power (FRAP), and phenanthroline. Those various antioxidant activities were compared to standard antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The extract demonstrated strong antioxidant activity across all methods, with low IC₅₀ values 253.6 , 1.68 , 32.68 and 4.11 µg/mL respectively, Overall, the results suggest that Eriobotrya japonica is a valuable plant with strong antioxidant properties, making it a potential candidate for various health-related applications.