Printed digital and conventional complete dentures require appropriate hygiene materials and methods to prevent the formation of biofilm, tartar, stains, inflammation, and odor. However, some hygiene materials can affect resin properties, such as surface roughness, which, when increased, can favor biofilm adhesion and development. In the literature, it has been reported that roughness values ≤ 0.2 µm are considered clinically acceptable; however, studies evaluating the effects of hygiene protocols on the roughness of printed resins are scarce. So, this study compared the roughness of 3D printed resins for denture bases (3DB) and teeth (3DT) to conventional resins for base (CB) and teeth (CT), after hygiene protocol use. Ten specimens of each material were subjected to brushing and immersion in water (EA, control), 0.25% sodium hypochlorite (EHS), or 0.15% triclosan (ET). Roughness was measured after obtaining the specimens (T0) and after simulating daily 6-minute brushing and daily 20-minute immersion for 1 (T1) and 3 years (T3), using a confocal laser microscope (µm). Data were analyzed using Generalized Estimating Equations (GEEs) and Wald test with Bonferroni adjustment (α=0.05). Roughness was influenced by the following interaction: resin × hygiene protocol × time (p=0.012). In T1, the average roughness (Sa - µm) of the 3DB (EA: 0.239; EHS: 0.258; and ET: 0.265) and 3DT (EA: 0.211; EHS: 0.202; ET: 0.248) resins was considered unacceptable. In T3, the roughness of the 3DB (EA: 0.183; EHS: 0.161; ET: 0.199) and 3DI (EA: 0.181; EHS: 0.173; ET: 0.188) resins decreased, possibly due to the removal of superficial layers of resin through brushing, exposing inner layers with less roughness. Conventional resin roughness remains within acceptable limits regardless of hygiene protocol. Compared to conventional resins, printing resins should be indicated for short-term situations, when associated with the proposed hygiene protocols. Hygiene protocols can be recommended for both conventional resins during the periods studied.