Chlorophyll content is a critical indicator of photosynthetic efficiency and plant vigour. Black Ginger (Kaempferia parviflora) is a new medicinal crop that is gaining popularity in Malaysia. This study aimed to evaluate the effects of different extraction methods and leaf developmental stages on chlorophyll content in Black Ginger (Kaempferia parviflora). Two extraction methods (dissolved and grinder) and three leaf stages (old, mature, and young) were tested, and chlorophyll a, chlorophyll b, and total chlorophyll (a+b) contents were determined. Results revealed that extraction methods significantly influenced chlorophyll a content (P < 0.0001), with the grinder method producing higher concentrations (1.5786 ± 0.0309 mg g⁻¹) compared to the dissolved method (1.1288 ± 0.0454 mg g⁻¹). In contrast, chlorophyll b content was not significantly affected by the extraction method (P = 0.46). Leaf developmental stages showed a strong effect on both chlorophyll a and total chlorophyll contents (P < 0.0001 and P = 0.0002, respectively), with young leaves recording the highest total chlorophyll content (3.3158 ± 0.1186 mg g⁻¹). Significant interactions between extraction methods and leaf stages were observed for chlorophyll a (P < 0.0001) and total chlorophyll (P = 0.01), highlighting the combined influence of methodological and physiological factors. These findings suggest that both extraction technique and leaf maturity must be considered for accurate chlorophyll determination in Black Ginger, with implications for optimizing physiological assessments and future studies on plant growth and bioactive compound evaluation.