Introduction:
This study examined how personality traits influence physiological markers of stress, focusing on the mediating role of perceived psychological stress in the link between personality and spontaneous eye-blinking behavior. Drawing on the Big Five framework, we explored how neuroticism and conscientiousness—traits that are closely tied to stress reactivity—relate to perceived stress and blink rate. Spontaneous blink rate (SBR), a dopaminergic and attentional marker, was used as a noninvasive physiological indicator of stress-related arousal.

Methods:
Eighty-six adults (74 females; M = 21.9 ± 2.5 years, range = 18–31) silently read a standardized Hebrew text while their blinks were recorded via webcam using custom Python-based software. Personality (BFI-2) and perceived stress (PSS-14) were assessed online approximately five months later to minimize reactivity. Correlational and path analyses tested direct and indirect associations between personality, perceived stress, and blink rate.

Results:
Neuroticism was positively related to perceived stress (β = 0.35; p < .001), which predicted a higher blink rate (β = 0.38; p < .001), supporting the mediation hypothesis. When modeled together, neuroticism showed a negative direct effect on blink rate (β = –0.30; p < .005), revealing a suppression effect. Conscientiousness indirectly predicted a lower blink rate through reduced stress (β = –0.26, p < .05; stress → blink rate, β = 0.29, p < .01). The model explained 12% of the variance in stress and 15% of the variance in blink rate.

Conclusions:
Perceived stress mediates the relationship between personality and blink dynamics, revealing dual pathways: neuroticism increases blinking via stress but directly reduces it through attentional mechanisms. Blink rate thus emerges as a sensitive psychophysiological marker linking personality and stress, with implications for noninvasive stress monitoring and personalized assessment in clinical and occupational contexts.